Lab Report on Effect of Enzymes Essay

ntroductionThe enzyme catalase is acquaint in cells in order to speed the disruption of enthalpy peroxide (H2O2), which is a toxic chemical to the human body. When hydrogen peroxide is confounded down, the end products are Water (H2O) and Oxygen (O2). In this report, the chemical reaction of catalase to hydrogen peroxide is being tried and true. Furthermore, the effects of temperature, concentration, and pH level on this reaction are being considered. We hypothesize that he experiments will show that Catalase speeds the breakdown of hydrogen peroxide to water and oxygen, and the oxygen will produce a larger bubbling effect in the solutions where the optimum conditions for enzymatic exertion are present. These optimum conditions should be in warm temperature, laid-back concentration, and high pH levels. First, the reaction of catalase to hydrogen peroxide is established with no supererogatory factors. Then, to subscribe to the effect of temperature, ternary ranges of tempe rature are introduced in the second experiment. Next, to study the effect of concentration, troika different levels of catalase are studied in the terzetto experiment. And finally, to study the effect of pH, solutions with three levels of hydrogen concentration are studyed in the fourth experiment, again with hydrogen peroxide and catalase.MethodsExperiment 1 To essay the catalase-hydrogen peroxide reaction with no additional factors, we trailed three taste renders to the 1 cm and 5 cm marks. Catalase was added to the 1 cm mark of analyze tube 1 and interrogatory tube 3, firearm test tube 2 was filled to the 1 cm mark with water. Hydrogen peroxide was added to the 5 cm mark in test tube 1 and test tube 2, piece a sucrose solution was used to fill test tube 3. All test tubes were mixed and we waited 20 seconds for bubbling. test tube 1 represented the experiment, magical spell test tube 2 was the negative control for hydrogen peroxide, and test tube 3 was the negative co ntrol for the catalase. Experiment 2 To test theeffect of temperature on enzyme activity, the solution created in test tube 1 of the previous experiment was recreated in three separate test tubes. test tube 1 was situated in an ice bath. Test 2 was placed in warm water. Test tube 3 was placed in boiling water. Experiment 3 To test the effect of concentration, three separate test tubes were set of to have three different concentrations of the reactant (catalase), while the substrate was consistent across all three test tubes.Test tube 1 was filled in the similar fashion as test tube 1 in the outset experiment. Test tube 2 was then filled to 2 cm with catalase and then to 7 cm with hydrogen peroxide. Test tube 3 was filled to 3 cm with catalase, and then to 8 cm with hydrogen peroxide. Experiment 4 Finally, to test the effect of pH Level on enzyme activity, three test tubes were again created. The concentration of catalase and hydrogen peroxide are again the same as in test tube on e in the first experiment. However, 2 cm of water correct to different pH levels were added. In test tube 1, 2 cm of water with pH of 3 is added to the 1 cm of catalase and 4 cm of hydrogen peroxide. In test tube 2, 2 cm of water adjusted to the pH adjusted to 7 was added to 1 cm of catalase and 4 cm of hydrogen peroxide. In test tube 3, 2 cm of water adjusted to the pH adjusted to 11 was added to 1 cm of catalase and 4 cm of hydrogen peroxide.DiscussionThe discussion section includes your interpretation of the results and provides the answer to the research hesitation described in the introduction. Specifically, discuss whether or not your hypotheses were supported. Also, include a comparison to previous studies, discuss the limitations of your study (briefly), and detail unexpected findings. Finally, ingeminate your conclusions and discuss the significance of your results in a broader context. Use the appropriate deform as described above.ReferencesThe references section is a list of all references cited in the text. Arrange references alphabetically according to author name, not chronologically. The name of the journals containing the cited paper should be written out in full. Town/city and democracy names should be provided for non-journal references. Each article reference should be tending(p) as in the following exampleAlfano J.R., Collmer A. (2004) Type III discrimination system effector proteins double agents in bacterial disease and coif defence. Annual Review Phytopathology, 42, 385414.Books or other non-serial publications which are quoted in the references must be cited as followsGage J.D., Tyler P.A. (1991) Deep-sea Biology A instinctive History of Organisms at the Deep-sea Floor. Cambridge University Press, Cambridge, UK 504 pp.Lester R.N., Hasan S.M.Z. (1991) Origin and domestication of the brinjal eggplant, genus Solanum melongena, from S. incanum, in Africa and Asia. In Hawkes J.G., Lester R.N., Nee M., Estrada N. (Eds), Solanaceae III Taxonomy, Chemistry, Evolution. Royal botanic Gardens, Kew London, UK 369387.